In contrast to target-based drug discovery, phenotypic screening discloses the effect of a compound on a biological system without revealing its mode of action or its interaction sites. Phenotypic screening has delivered several new valuable drugs, however, the major challenge remains target deconvolution (1). In recent years, “omics” techniques and bioinformatics have brought significant advances to target identification in drug discovery phenotypic screening. However, such analyses can become ...
Ref.-Nr.: 0903-5658-LC; 0903-5696-LC; 0903-5692-LC
Imaging and Microscopy
Super-resolution microscopy critically relies on the availability of appropriate fluorophores. For single-molecule localization microscopy (SMLM), fluorophores are needed that stochastically switch between bright (on) and dark (off) states. This phenomenon is also called fluorescence blinking. For stimulated emission depletion microscopy (STED) the dyes must be photo-switchable.
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Diagnostics
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Extremely sensitive, reliable and fast novel KDAC assay, which can be performed in a continuous format. This novel KDAC assay is based on Firefly luciferase harboring an acetylation on an essential active site lysine, incorporated by genetic code expansion. We furthermore offer a bacterial selection system for KDACs, which uses a reporter enzyme inactivated by lysine modifications at an essential active site residue, thereby linking deacetylase activity to a selectable output.
Medicine : Therapeutics
Medicine : Diagnostics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related
Biomarkers of ageing have long been sought after to help assess the biological age and general health status of individuals. Considerable efforts have been invested to identify such biomarkers, including physiologic readouts, metabolic parameters, glycomic profiles and others. Nevertheless, markers with strong predictive power have remained elusive.
Work in model organisms has identified several conserved signaling pathways that extend life span, such as reduced insulin/IGF signaling and ...
Novel method for analysis of complex carbohydrate structures by ion-mobility mass spectrometry (IM-MS)
Carbohydrates form one of the major classes of biological macromolecules in living organisms, but structural analysis and the quality control of carbohydrate structures represent a major challenge due to the frequent occurrence of isomers that are difficult to distinguish using established techniques.
Changes in protein glycosylation are frequently identified in the context of diseases. For example, alterations in N-glycan profiles from blood plasma have been found to correlate with pathological ...
A simple, one-step assay for reliable measurements of NAD(H) and NADP(H) in complex samples, with various applications such as enzymatic assays, clinicals tests, and as point of care tests in the field of ageing.